Reporter

Part:BBa_K223060

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


5MT Inverter-GFP Reporter and Lac Promoter

Identical to part BBa_K223059 except with a Lac promoter. This part can be used to test the efficiency of the 5MT inverter system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 742
    Illegal suffix found in sequence at 576
    Illegal SpeI site found at 891
    Illegal PstI site found at 905
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 742
    Illegal SpeI site found at 577
    Illegal SpeI site found at 891
    Illegal PstI site found at 591
    Illegal PstI site found at 905
    Illegal NotI site found at 235
    Illegal NotI site found at 584
    Illegal NotI site found at 748
    Illegal NotI site found at 898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 577
    Illegal EcoRI site found at 742
    Illegal XbaI site found at 757
    Illegal SpeI site found at 891
    Illegal PstI site found at 905
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 742
    Illegal XbaI site found at 244
    Illegal XbaI site found at 757
    Illegal SpeI site found at 577
    Illegal SpeI site found at 891
    Illegal PstI site found at 591
    Illegal PstI site found at 905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1580
    Illegal SapI.rc site found at 423


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